Composite

Part:BBa_K2976013:Design

Designed by: Jiatong Chen   Group: iGEM19_CPU_CHINA   (2019-09-05)


TLR2-P2A-TLR1-T2A-CD14


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5212
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 552
    Illegal BamHI site found at 3226
    Illegal XhoI site found at 5243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2541
    Illegal BsaI site found at 5063


Design Notes

Codon optimization


Source

NCBI, with codon optimization.

References